Project Description
Protein A affinity chromatography medium by EPRUI is based on the monodisperse porous poly (methyl methacrylate) (PMMA) microspheres as the matrix, and genetically modified protein A as the ligand, which ensures its high affinity binding of monoclonal antibodies and Fc fragment containing recombinant proteins (macromolecules).
Our protein A chromatography media has high mechanical strength and good pH stability. Even at a high flow rate, the beads still maintained a high dynamic binding capacity, meeting the demands from laboratory preparation to industrial production.
Applications
EPRUI Protein A media was successfully applied in purification of hIgG from human serum.
Experimental：
Column: 7 mm×25 mm
Sample: human serum, 5 mL
Equilibration Buffer: 20mM PBS, pH 7.0 0.15 M NaCl
Elution: 0.1 M Glycine, pH 3.0
Residence time: 4 min
Features & Advantages
Features
- Highly uniform particle size
- Good mechanical strength
- Higher flow rate
- High loading capacity
- Better alkaline stability
Advantages
- Good reproducibility ,easy to pack
- Higher pressure resistance, able to pack preparative column in large scale
- Higher productivity
- Cost effective
- Clean with 0.1-0.5M NaOH
Product List
| Matrix | Monodisperse PMMA Microspheres |
| Diameter | 50um |
| Ligand | Recombinant Protein A |
| hIgG Dynamic Binding Capacity | >35mg/ml(4 min residence time) |
| Linear Flow Rate | 300-700cm/h |
| Maximum Pressure | 72.5 psi(5 bar,0.5Mpa) |
| PH Stability | 3-12 |
| Temperature | 4-40℃ |
| CIP Reagent | 0.1-0.5M NaOH |
| Storage | 20% ethanol, 2-8℃ |
Prepacked Column Specification
| Dimension | 7mm*25mm |
| Cartridge | Polypropylene(PP) |
| Column volume | 1ml |
| hIgG Dynamic Binding Capacity | >35mg/ml(4 min residence time) |
| Flow Rate | 0.25-1.0ml/min |
| Maximum Pressure | 43.5 psi(3 bar, 0.3Mpa) |